- Research article
- Open Access
Determination of toxic (Pb, Cd) and essential (Zn, Mn) metals in canned tuna fish produced in Iran
© Hosseini et al. 2015
- Received: 17 July 2013
- Accepted: 5 August 2015
- Published: 11 August 2015
Metal pollution of waterways directly affects human health and can impact the food chain. Seafood living in polluted water can accumulate trace metals. The purpose of this study was to analyze the toxic metals Pb and Cd and the dietary essential metals Zn and Mn in 120 cans of tuna species from four different brands processed in Iran and purchased in 2012.
The mean level of metals for each brands of canned fish obtained in mg/kg were as follows: yellowfin tuna (Pb: 0.19 ± 0.015, Zn: 5.77 ± 4.17, Mn: 0.08 ± 0.07, Cd: 0.15 ± 0.12), Kilka (Pb: 0.95 ± 0.88, Zn: 30.47 ± 29.82, Mn: 1.01 ± 0.73, Cd: 0.07 ± 0.05), Kawakawa (Pb: 0.28 ± 0.23, Zn: 6.77 ± 5.21, Mn: 0.17 ± 0.12, Cd: 0.12 ± 0.09), longtail tuna (Pb: 1.59 ± 1.56, Zn: 7.44 ± 6.11, Mn: 0.04 ± 0.03, Cd: 0.06 ± 0.04). Pb, Zn and Cd levels were generally higher than the FAO/WHO permissible limits (Pb: 0.50 mg/kg, Zn: 50.0 mg/kg and Cd: 0.50 mg/kg) and the European Union acceptable dietary limits.
Based on the United States Environmental Protection Agency health criteria, there is no health risk associated with Mn concentration in the samples analyzed. The limits of detection of the method for Pb, Zn, Mn and Cd in mg/kg were 0.01, 0.5, 0.01 and 0.01, respectively. The result of the one-way analysis of variance suggested significant variations (p < 0.05) in the concentration of the metals in the different types of canned fish with the following being outside of compliance levels.
- Canned fish
- Food safety
During the last few decades there has been a growing interest in determining the level of toxic metals in marine and fresh water environments with additional emphasis on the measurement of contamination levels in the food supply, particularly fish [1–12] including canned fish. For example, Tuzen and Soylak determined the Cu, Zn, Mn, Fe, Se, Al, Cr, Ni, Pb and Cd concentrations in canned fish marketed in Turkey . Mol analyzed the levels of Fe, Zn, Cu, Cd, Sn, Hg and Pb in canned bonito, sardines, and mackerel , canned tuna fish  and canned anchovies and canned rainbow trouts  produced in Turkey. Hosseini et al. determined Hg, Se and Sn concentrations in canned fish marketed in Iran  while Mahalakshmi et al. determined Al, Cd, Pb and Hg in canned tuna fish available in Canada and India . Trace metals are important for both their necessity and toxicity. Some elements like Mn and Zn are essential functional and structural elements in biological systems [13–18] often catalyzing reactions by binding to substrates, there by favoring various reactions such as the mediation of oxidation–reduction reactions, or redox reactions, through reversible changes in the oxidation state of the metal ions [15, 16, 19–21]. For Mn and Zn, often called micronutrients, there are fixed allowed levels that provide for an adequate dietary intake according to the World Health Organization (WHO). In adults from 5.0 to 22.0 mg are recommended for Zn and from 2.0 to 20.0 mg are recommended for Mn [22, 23]. At high concentrations, Zn causes nephritis, anuria and extensive lesions in the kidneys [24, 25].
Pb and Cd are very toxic to humans. They are only tolerated at extremely low concentrations and excesses are associated with many adverse health effects [12, 26]. They may injure the kidney and cause symptoms of chronic toxicity, including impaired organ function, poor reproductive capacity, hypertension, tumors and hepatic dysfunction . Moreover, Pb can also affect brain function by interfering with neurotransmitter release and synapse formation. Exposure to Pb has been associated with reduced IQ, learning disabilities, slow growth, hyperactivity, antisocial behaviors and impaired hearing . Generally Pb-poisoning is ranked as the most common environmental health hazard .
Use of Cd in agriculture and industry has been identified as a major source of its wide dispersion in the environment and food. The major route of exposure to Cd for non-smokers is via food; the contribution from other pathways to total uptake is small . Certain marine vertebrates contain markedly elevated Cd concentrations .
Trace and toxic metals may contaminate fish, mainly during the growth phase but also, sometimes, due to contamination during transportation and storage. Therefore, most countries monitor the levels of toxic metals in seafood [5, 30, 31]. Levels of toxic metals in fish depend on many factors like the duration of exposure of fish to contaminants in the water, the feeding habit of the fish, the concentrations of contaminants in the water column, and sometimes to the water chemistry, contamination of fish during handling and processing, age, sex, weight, season, fish species, catching area, transportation, and storage, etc. [21, 32, 33]. According to Taha’n et al., the pH of the canned product, the quality of the lacquer coatings of canned products, oxygen concentration in the headspace, quality of the coating and storage place may also affect metal levels in canned fishes .
Many species of commercially caught marine fish, especially in Iran, are canned, thus making them more available for human consumption to those living far from the sea .
Tuna fish are long living organisms prone to accumulate pollutants. Canned tuna fish is eaten regularly in many countries including Iran (globally over the 10 × 1010 tonne per year) [12, 36–39]. In this study the levels of the toxic and essential metals (Pb, Zn, Mn and Cd) in four different commercial types of canned tuna fish (longtail tuna, Kawakawa, Kilka and yellowfin tuna) commonly consumed in Iran were determined using GFAAS . This study will help to generate the data needed for surveillance programs aimed at ensuring the safety of the food supply and minimizing human exposure to toxic metals.
During the year 2012, 120 samples (185 g each) of four different commercial types of canned fish commonly consumed in Iran (30 samples for each type: “Famila Co.” (Tehran): yellowfin tuna (YT) (Thunnus albacares); “Shilaneh Co.” (Qazvin): common Kilka (CK) (Clupeonella cultriventris caspia); “Pars Tuna Co.” (Bushehr): Kawakawa (Ka) (Euthynnus affinis), and “Hiltune Co.” (Tehran): longtail tuna (LT) (Thunnus tonggol)) were purchased at markets within Tehran.
All glassware was cleaned by soaking overnight in 10 % nitric acid, followed by rinsing with distilled water. The acids used for wet digestion were of analytical reagent (Merck, Darmstadt, Germany) grade, while the distilled water was further deionized. The blank values were below the detection limits of the instrument. Working standards were made from the stock by dilution of the measured aliquots with 1.0 M nitric acid. Each sample was analyzed in triplicate and the results, which mostly agreed within ±1.0 %, were averaged. A reagent blank determination was carried out with every batch of 10 samples.
Recovery of various metals from canned fish samples
One-way analyses of variance (ANOVA) and Tukey’s test were used to determine whether Pb, Zn, Mn and Cd concentrations varied significantly between specimens, with probability values less than 0.05 (p < 0.05) considered statistically significant. The statistical calculations were done using SPSS 15.0 version (SPSS Inc., Chicago, IL, USA) statistical package.
Metals content (mg/kg) for various species of canned fish*
Mean ± SD
Mean ± SD
Mean ± SD
Mean ± SD
0.19 ± 0.015a
0.95 ± 0.88c
0.28 ± 0.23b
1.59 ± 1.56d
5.77 ± 4.17a
30.47 ± 29.82d
6.77 ± 5.21b
7.44 ± 6.11c
0.08 ± 0.07b
1.01 ± 0.73d
0.17 ± 0.12c
0.04 ± 0.03a
0.15 ± 0.12d
0.07 ± 0.05b
0.12 ± 0.09c
0.06 ± 0.04a
The comparative levels based on the average of Pb, Zn, Mn and Cd in the various types of canned fish shows that the average concentrations of Pb in LT are higher than the other species by 1.7, 5.7 and 8.3 times as compared to CK, Ka and YT, respectively. The average Zn content in CK was much higher than other the canned fish, as compared to LT, Ka and YT, respectively. Similar behavior is shown by Mn. The average Pb content in LT was much higher than other the canned fish, as compared to YT, Ka and CK, respectively. The average Cd content in LT was about 3 times lower than YT.
Comparison of present mean values in specimens with other studies result
Yellowfin tuna, Kilka, Kawakawa, longtail tuna
Anguilla Anguilla, Mugil cephalus, Oreochromis niloticus
Otolithes ruber, Pampus argenteus, Parastromateus niger, Scomberomorus commerson, Onchorynchus mykiss
Canned salmon, sardine and tuna fish
Turkeyf, g, h
Canned anchovies, canned rainbow trout
Several organizations, such as the FAO and WHO, provide guidelines on the intake of trace elements by humans. The provisional tolerable weekly intake (PTWI) recommended by the Joint FAO/WHO Expert Committee (1972) for Cd and Pb are 7 μg Cd/kg body weight per week and 25 μg Pb/kg body weight per week respectively [45, 46]. Therefore, the provisional tolerable weekly intake of Cd and Pb for a 60 kg adult (halfway between the 70 kg male and 50 kg female normally taken as the standard) was estimated to be 420 and 1500 μg/person/week, respectively. The maximum Cd level found in this study was 0.37 mg/kg and therefore a 60 kg adult could safely consume 1135 g portions of fish per week with that level. The maximum Pb concentration observed was 5.50 mg/kg and thus consumption of more than 272 g of fish per week exceeds the tolerable weekly intake of Pb. Toxic metal concentrations in this study were considerably higher than those found previously found in fish by Ikem and Egeibo; Iwegbue et al.; Mol; Boadi et al. and Mahalakshmi et al. [5, 11, 25, 33, 38].
The results of this study suggested that significant differences existed in the element concentrations across four different species of canned fish. Also, analytical data obtained from this study shows that the metal concentrations for the varieties of canned fishes especially Cd and Pb were generally higher than the FAO/WHO, FDA and U.S. EPA recommended limits for fish [47, 48]. Therefore both low-risk groups (adolescents and adults) and high-risk groups (pregnant mothers and children) must, based on the results obtained, reduce their consumption of canned fish as frequent consumption may result in bioaccumulation of the metals and increased health risks. Globally, further reduction in the levels of environmental contaminants emanating from power plants and other industrial emissions and effluent discharges are needed to reduce contaminant inputs into the aquatic environment. More research and assessments of seafood quality is needed in many countries to provide more data and help safeguard the health of humans. Therefore, it was concluded that toxic metals in canned fish must be monitored comprehensively and periodically with respect to the consumer health.
The authors are grateful to the University of Tehran for providing facilities to conduct and complete this study.
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- Tuzen M, Soylak M. Determination of trace metals in canned fish marketed in Turkey. Food Chem. 2007;101:1378–82.View ArticleGoogle Scholar
- Ruelas-Inzunza J, Meza-Lopez G, Paez-Osuna F. Mercury in fish that are of dietary importance from the coasts of Sinaloa (SE Gulf of California). J Food Comp Anal. 2008;21(3):211–8.View ArticleGoogle Scholar
- Bhattacharyya S, Chaudhuri P, Dutta S, Chandra SS. Assessment of total mercury level in fish collected from East Calcutta Wetlands and Titagarh sewage fed aquaculture in West Bengal, India. Bull Environ Contam Toxicol. 2010;84:618–22.View ArticleGoogle Scholar
- Sae S, Dehkordi S, Fallah AA, Nematollahi A. Arsenic and mercury in commercially valuable fish specied from the Persian Gulf: influence of season and habitat. Food Chem Toxicol. 2010;48(10):2945–50.View ArticleGoogle Scholar
- Mol S. Levels of heavy metals in canned bonito, sardines, and mackerel Produced in Turkey. Biol Trace Elem Res. 2011;143:974–82.View ArticleGoogle Scholar
- Mol S. Levels of selected trace metals in canned tuna fish produced in Turkey. J Food Comp Anal. 2011;24:66–9.View ArticleGoogle Scholar
- Mol S. Determination of trace metals in canned anchovies and canned rainbow trouts. Food Chem Toxicol. 2011;49(2):348–51.View ArticleGoogle Scholar
- Ordiano-Flores A, Galván-Magaña F, Rosiles-Martínez R. Bioaccumulation of Mercury in Muscle Tissue of yellowfin tuna, Thunnus albacares, of the Eastern Pacific Ocean. Biol Trace Elem Res. 2011;144(1-3):606–20.View ArticleGoogle Scholar
- Rezayi M, Esmaeli AS, Valinasab T. Mercury and selenium content in Otolithes ruber, and Psettodes erumei from Khuzestan Shore, Iran. Bull Environ Contam Toxicol. 2011;86:511–4.View ArticleGoogle Scholar
- Hosseini SV, Aflaki F, Sobhanardakani S, Tayebi L, Babakhani Lashkane A, Regenstein JM. Analysis of mercury, selenium and tin concentrations in canned fish marketed in Iran. Environ Monit Assess. 2013;185(8):6407–12.View ArticleGoogle Scholar
- Mahalakshmi M, Balakrishnan S, Indira K, Srinivasan M. Characteristic levels of heavy metals in canned tuna fish. J Toxicol Environ Health Sci. 2012;4(2):43–5.View ArticleGoogle Scholar
- Emami Khansari F, Ghazi-Khansari M, Abdollahi M. Heavy metals content of canned tuna fish. Food Chem. 2005;93:293–6.View ArticleGoogle Scholar
- Narin I, Soylak M, Elci L, Dog˘an M. Determination of trace metal ions by AAS in natural water samples after preconcentration of pyrocatechol violet complexes on an activated carbon column. Talanta. 2000;52:1041–6.View ArticleGoogle Scholar
- Soylak M, Saracog˘lu S, Tuzen M, Mendil D. Determination of trace metals in mushroom samples from Kayseri, Turkey. Food Chem. 2005;92:649–52.View ArticleGoogle Scholar
- Ibrahim HS, Ibrahim MA, Samhan FA. Distribution and bacterial bioavailability of selected metals in sediments of Ismailia Canal, Egypt. J Hazard Mater. 2009;168:1012–6.View ArticleGoogle Scholar
- Ibrahim M, Shaltout AA, Atta DE, Jalbout AF, Soylak M. Removal of COOH, Cd and Pb using water hyacinth: FTIR and flame atomic absorption study. J Iran Chem Soc. 2009;6:364–72.View ArticleGoogle Scholar
- Sounderajan S, Kumar GK, Udas AC. Cloud point extraction and electrothermal atomic absorption spectrometry of Se (IV) –3,3 diaminobenzidine for the estimation of trace amounts of Se (IV) and Se (VI) in environmental water samples and total selenium in animal blood and fish tissue samples. J Hazard Mater. 2010;175(1-3):666–72.View ArticleGoogle Scholar
- Ghaedi M, Shokrollahi A, Kianfar AH, Mirsadeghi AS, Pourfarokhi A, Soylak M. The determination of some heavy metals in food samples by flame atomic absorption spectrometry after their separation-preconcentration on bis salicyl aldehyde, 1,3 propan diimine (BSPDI) loaded on activated carbon. J Hazard Mater. 2008;154:128–34.View ArticleGoogle Scholar
- Hlihor RM, Gavrilescu M. Removal of some environmentally relevant heavy metals using low-cost natural sorbents. Environ Eng Manag J. 2009;8:353–72.Google Scholar
- Kadi MW. Soil pollution hazardous to environment: a case study on the chemical composition and correlation to automobile traffic of the roadside soil of Jeddah city, Saudi Arabia. J Hazard Mater. 2009;168:1280–3.View ArticleGoogle Scholar
- Mendil D, Demirci Z, Tuzen M, Soylak M. Seasonal investigation of trace element contents in commercially valuable fish species from the Black Sea, Turkey. Food Chem Toxicol. 2010;48(3):865–70.View ArticleGoogle Scholar
- World Health Organization (WHO). Health criteria and other supporting information. In: Guidelines for drinking water quality. 2nd ed. Geneva: World Health Organization; 1995. p. 31–388.Google Scholar
- Tarley CRT, Coltro WKT, Matsushita M, De Souza NE. Characteristic levels of some heavy metals from Brazilian canned sardines (Sardinella brasiliensis). J Food Comp Anal. 2001;14:611–7.View ArticleGoogle Scholar
- Abou-Arab AAK, Ayesh AM, Amra HA, Naguib K. Characteristic levels of some pesticides and heavy metals in imported fish. Food Chem. 1996;57(4):487–92.View ArticleGoogle Scholar
- Iwegbue CMA, Nwajei GE, Arimoro FO, Eguavoen O. Characteristic levels of heavy metals in canned sardines consumed in Nigeria. Environmentalist. 2009;29:431–5.View ArticleGoogle Scholar
- Castro-González MI, Méndez-Armenta M. Heavy metals: implications associated to fish consumption. Environ Toxicol Pharmacol. 2008;26(3):263–71.View ArticleGoogle Scholar
- Dahiya S, Karpe R, Hegde AG, Sharma RM. Lead, cadmium and nickel in chocolate and candies from suburban areas of Mumbai, India. J Food Comp Anal. 2005;18:517–22.View ArticleGoogle Scholar
- Goyer RA. Toxic effects of metals. In: Amdur MO, Douli J, Klansmen CD, editors. Caserrett and Doull’s Toxicology: The Basic Science of Poisons. Fourthth ed. New York: Pergamon Press; 1991. p. 623–80.Google Scholar
- Jarup L, Berglund M, Elinder CG, Nordberg G, Vahter M. Health effects of cadmium exposure- a review of the literature and a risk estimate. Scand J Work Environ Health. 1998;24:1–52.View ArticleGoogle Scholar
- Ashraf W. Levels of selected heavy metals in tuna fish. Arab J Sci Eng. 2006;31(1A):89–92.Google Scholar
- Ganjavi M, Ezzatpanah H, Givianrad MH, Shams A. Effect of canned tuna fish processing steps on lead and cadmium contents of Iranian tuna fish. Food Chem. 2010;118:525–8.View ArticleGoogle Scholar
- Kagi JH, Schaffer A. Biochemistry of metallothionein. Biochem. 1998;27:8509–15.View ArticleGoogle Scholar
- Boadi NO, Twumasi SK, Badu M, Osei I. Heavy metal contamination in canned fish marketed in Ghana. Amer J Sci Indus Res. 2011;2(6):877–82.Google Scholar
- Taha’n JE, Sanchez JM, Granadillo VA, Cubillan HS, Romero RA. Concentration of total Al, Cr, Cu, Fe, Hg, Na, Pb, and Zn in commercial canned seafood determined by atomic spectrometric means after mineralization by microwave heating. J Agric Food Chem. 1995;43:910–5.View ArticleGoogle Scholar
- Dabeka RW, McKenzi AD, Albort RH. Atomic absorption spectrophotometric determination of tin in canned foods, using nitric acid-hydrochloric acid digestion and nitrous oxide-acetylene flame: collaborative study. J Assoc Off Anal Chem. 1985;68:209–13.Google Scholar
- Voegborlo RB, El-Methnani AM, Abedin MZ. Mercury, cadmium and lead content of canned tuna fish. Food Chem. 1999;67:341–5.View ArticleGoogle Scholar
- Ashraf W, Seddigi A, Abulkibash A, Khalid M. Levels of selected metals in canned fish consumed in Kingdom of Saudi Arabia. Environ Monit Assess. 2006;117:271–9.View ArticleGoogle Scholar
- Ikem A, Egeibor NO. Assessment of trace elements in canned fishes (mackerel, tuna, salmon, sardines and herrings) marketed in Georgia and Alabama (United States of America). J Food Comp Anal. 2005;18:771–87.View ArticleGoogle Scholar
- Lourenço HM, Afonso C, Martin MF, Lino AR, Nunes ML. Levels of toxic metals in canned seafood. J Aqua Food Prod Technol. 2004;13(3):117–25.View ArticleGoogle Scholar
- European Union (EU). Commission regulation as regards heavy metals. Amending Regulation 466/2001; 2005., no 78/2005.Google Scholar
- Eboh L, Mepba HD, Ekpo MB. Heavy metal contaminants and processing effects on the composition, storage stability and fatty acid profiles of five common commercially available fish species in Oron Local Government, Nigeria. Food Chem. 2006;97(3):490–7.View ArticleGoogle Scholar
- Canli M, Atli G. The relationships between heavy metal (Cd, Cr, Cu, Fe, Pb, Zn) levels and the size of six Mediterranean fish species. Environ Pollut. 2005;121:129–36.View ArticleGoogle Scholar
- Turkmen M, Turkmen A, Tepe Y, Tore Y, Ates A. Determination of metals in fish species from Aegean and Mediterranean Seas. Food Chem. 2009;113:233–7.View ArticleGoogle Scholar
- Ministry of Agriculture, Forestry and Fisheries (MAFF). Monitoring and surveillance of non-radioactive contaminants in the aquatic environment and activities regulating the disposal of wastes at sea, Aquatic Environment Monitoring; 1995. Report No. 44.Google Scholar
- Food and Agriculture/World Health Organization (FAO/WHO). Evaluation of certain food additives and the contaminants mercury, cadmium and lead. Geneva: WHO Technical Report Series; 1972. p. 505.Google Scholar
- Anonymous: IARC (International Agency for Research on Cancer). Cadmium and cadmium compounds (Group 1), IARC monographs.58. Lyon, France: International Agency for Research on Cancer; 1993.Google Scholar
- Anonymous. Joint FAO/WHO expert committee on food additives, Sixty-first Meeting Rome, 10-19 June 2003.Google Scholar
- Aucoin J, Blanchard R, Billiot C. Trace metals in fish and sediments from Lake Boeuf, South Eastern Louisiana. Microchem J. 1999;62:299–307.View ArticleGoogle Scholar
- Yilmaz F. The comparison of heavy metal concentrations (Cd, Cu, Mn, Pb, and Zn) in tissues of three economically important fish (Anguilla anguilla, Mugil cephalus and Oreochromis niloticus) inhabiting Köycegiz Lake-Mugla (Turkey). Turk J Sci Tech. 2009;4(1):7–15.Google Scholar
- Sobhanardakani S, Tayebi L, Farmany A. Toxic metal (Pb, Hg and As) contamination of muscle, gill and liver tissues of Otolithes rubber, Pampus argenteus, Parastromateus niger, Scomberomorus commerson and Onchorynchus mykiss. World Appl Sci J. 2011;14(10):1453–6.Google Scholar
- Sobhanardakani S, Tayebi L, Farmany A, Cheraghi M. Analysis of trace elements (Cu, Cd and Zn) in muscle, gill and liver tissues of some fish species using anodic stripping voltammetry. Environ Monit Assess. 2012;184(11):6607–11.View ArticleGoogle Scholar