Evaluation of chromosomal aberrations caused by air pollutants in some taxi drivers from two polluted districts of urban Tehran and its comparison with drivers from rural areas of Lahijan: a pilot study
© Taghizadeh et al.; licensee BioMed Central. 2014
Received: 16 June 2014
Accepted: 6 December 2014
Published: 19 December 2014
Chromosome instability is the most common form of genomic instability. Genomic instability can lead to tumorogenesis. High level of chromosomal aberrations in peripheral blood lymphocytes can be used as a biomarker for cancer. Air pollution is one of the most important factors that cause chromosomal instability (CIN). In this comparative study we used classic Cytogenetic technique to analyze the effects of air pollutants on chromosome stability. We collected peripheral blood from 30 taxi drivers of two polluted districts (districts 6 and 7) in Tehran and 30 taxi drivers from rural areas of Lahijan, north of Iran.
Comparison of the level of chromosome breakage in the two groups showed an increased level of chromosome breakage in the drivers from polluted districts of Tehran, although not significant, using Fisher exact test (p-value = 0.300). However, the overall chromosome aberration rate (including both chromosome and chromatid gaps), the difference was significant using Chi-square test (p-value = 0.012).
An increased level of chromosome aberration was present in the drivers from polluted districts of Tehran compared to drivers from non-polluted areas in Lahijan.
Air pollution is concerned with many ill effects on health including pulmonary and cardiovascular diseases, cancer as well as increased mortality . Additionally, a relationship between intrauterine growth retardation and high exposure to air pollutants during pregnancy has been reported . This has been demonstrated in children and pregnant mothers living in industrial regions. Chromosomal aberrations and gene mutations lead to different kinds of diseases including cancer, particularly Leukemia . The damaging pollutants include poly aromatic hydrocarbons (PAH), methylbenzene, inhaled Ozone, stress oxidative damage of oxygen (ROS), nitrogen (RNS), and persistent particulate matter with size less than 2.5 micron .
PAHs are widespread environmental toxicants in ambient air produced from the partial combustion of fossil fuels. Different studies have evaluated the genetic damage in residents in polluted cities and compared them with those living in rural areas. They have shown that air pollution can have carcinogenic effect causing chromosomal aberrations in adults .
Adverse health effects are inflicted through physical and chemical properties of air born particulate matter (PM). These pollutants may induce either direct or indirect damage to biological macromolecules, including DNA. Several studies have indicated that pollutants have destructive effect on chromosomes and other important biomolecules . There are both exogenous physical agents and endogenous chemical genotoxic agents, both of which cause DNA damage including double strand and single strand breaks. Besides, ultraviolet can induce both interstrand and intrastrand crosslinks. They can all contribute towards formation of DNA adducts. Defect in repair and replication mechanisms are two outcomes of DNA damage. Such DNA defects can cause mutation or chromosomal aberrations leading to genomic instability . The common damages on chromosomes are breaks, gaps, translocations and acentric fragments .
Genomic instability is recognized as a major stimulating force of tumorogenesis . The final goal of cell division for most non-cancerous somatic cells is to accurately duplicate the genome and then evenly divide into the two daughter cells. However any deregulation of cell cycle can lead to aberrant cells. Accumulation of these genetic alterations due to failure in regulation of cell division, the imbalance between cell growth and death can eventually lead to cancer . There are different forms of genomic instability. The most common form is called chromosomal instability (CIN), leading to high rate of changes in chromosome structure and number, which were first observed more than a hundred years ago .
The accepted technique for biological monitoring of genetic damage to somatic cells is conventional cytogenetic analysis of peripheral blood lymphocytes, which has been adapted internationally since 1970. It has the advantage of determining the DNA damage by physical and chemical clastogenic agents in the occupational situation. Today, chromosomal aberration in human peripheral lymphocytes is recognized as a valuable biomarker of effect. It is generally accepted that a high frequency of chromosomal aberrations in peripheral lymphocytes is predictive of an increased risk of cancer .
The air pollution in Tehran
Materials and method
Heparinized blood was collected from the divers. Blood cultures were established within 24 h of specimen collection. Cell cultures were cultivated for 48 h at 37°C in RPMI 1640 with 0.5 cc 0f PBS and 1% phytohemagglutinin. Two duplicate cultures were set up from each sample. After 24 h we added 50 λ Colcemid to each tube and then after 1 h incubation both cultures were harvested using a classic technique that include centrifugation, treatment with hypotonic solution of KCL for 20 min followed by repeated fixation with methanol/acetic acid(3/1) .
Frequency of different types of chromosome aberration in case and control groups with aberrant cells (X 2 -test, P-value = 0.012)
Data were analyzed using SPSS 11.5 software. Data are shown as frequency and percentage in study groups. X2test was carried out. P value of less than 0.05 was considered as statistically significant.
Frequency of aberrant cells with breaks
Aberrant No. of cells with breaks
Total No. of cells scored
Rate of aberrant cells/1000 (CI95%)
The air quality of Tehran has deteriorated in recent years. In particular during autumn and winter seasons the concentration of pollutants increase because of the inverted air phenomenon . The effect of air pollution on morbidity and mortality has become a public health concern . Numerous studies have related high levels of air pollutants to chromosome instability. Genomic instability is a principal agent that can lead to cancer . Some studies have indicated that air pollution causes epigenetic alterations, mitochondrial and nuclear damage. Such genetic alterations can account for behavior disorder in Autistic children. These genetic changes are both microscopic and submicroscopic that can be detected using micro array analyses and DNA sequencing . Similar studies have shown different panel of methylation in DNA in industrial residents exposed to pollutants in comparison with rural residents. They used polymerase chain reaction (PCR) and pyrosequencing to investigate these changes on template of DNA methylation with biomarkers such as LINE-1, P53, IL-6, and P16. Several of these methylation markers showed differences between industrial workers and rural controls . Harmful effects of air pollution on health have been demonstrated in several investigations. It can lead to several diseases such as cancer, especially lung cancer. Stress oxidative damage results in inflammation and increasing coagulopathy activities in blood . Several studies have used cytogenetic parameters including chromatid and chromosome aberrations to demonstrate the adverse effect of air pollutants on genomic stability. Both classic and molecular cytogenetic techniques have been used . Few studies have compared conventional cytogenetic method with FISH and Micronucleus (MN) techniques and found noticeable differences between conventional cytogenetic and the latter techniques . The FISH technique using whole chromosome painting probes is as a rapid and sensitive method for detecting structural rearrangements, in particular reciprocal translocations. Such rearrangements can be inherited and passed on to the next generation if affecting the germ cells. The micronucleus technique is also an efficient method for the detection of aneuploidies and large number of cells can be screened . Using of FISH and Micronucleus (MN) can be useful to detect translocations and aneuploidies in the nuclei . In some studies focusing on the different seasons, significant differences in chromosome aberrations have been found during summer and winter . In a study by Rossner et al. using classic Cytogenetic technique, they determined the level of vitamins A, C, E, and folate in both case and control groups and did not find any significant difference between the two groups . The level of chromosome aberration was not significant either.
In this study using classic Cytogenetic technique we demonstrated a significantly increased level of chromosome aberration (gaps and breaks) in the drivers from polluted districts of Tehran compared to drivers from non-polluted areas in North of Iran. However this is a preliminary study and such an investigation should be carried out on a larger sample size.
An increased level of chromosome breakage was present in the drivers from polluted districts of Tehran compared to drivers from non-polluted areas in Lahijan. Based on these results and the importance of air pollution on morbidity and mortality, it is highly recommended that further similar studies on a larger sample size from other polluted areas of Tehran should be carried out for more conclusive results. Use of FISH and Micronucleus techniques are also highly recommended.
The authors gratefully acknowledge all the participants who took part in this study. We thank the Student’s Research Committee of the University of Social Welfare and Rehabilitation Sciences for funding this project. Our special thanks to the Center of quality control of air in Tehran and Guilan state for providing the data on level of pollutions in the studied areas. We are grateful to Mr. Hajipour from Tehran taxi bus services, Mr. Ali Taghizadeh from Guilan province, and Mrs Fatemeh Ostaresh for helping with collecting blood samples from the individuals. We are thankful to Miss Susan Banihashemi and Mr. Eiman Bagheizadeh for their assistance in the laboratory work.
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