- Research article
- Open Access
Fungal air quality in hospital rooms: a case study in Tehran, Iran
© Azimi et al.; licensee BioMed Central Ltd. 2013
Received: 31 July 2012
Accepted: 11 August 2013
Published: 19 December 2013
Fungi are usually presented in indoor environments and cause of many diseases. The aim of this descriptive study was to investigate the level of fungal contamination in hospital rooms. Sampling was conducted with an Andersen one-stage viable impactor (Quick Take-30) and counting plates containing a fungus-selective medium. A total of 120 air samples from ten hospital environments were performed. Airborne fungi concentrations were determined 72-120 hours after sampling. Total mean concentration of detected fungi in the hospital rooms was 55 ± 56 (mean ± SD) cfu/m3. The findings of the fungal concentration in the various hospital rooms revealed different levels of contamination: the lowest mean counts (37 ± 17 cfu/m3) were observed in NS 1 (Nursing Stations 1), and the highest (97 ± 217 cfu/m3) were reported in Orthopedics Operating Room (OOR). The most common fungal genus isolated were Penicillium (70%), Aspergillus (14%), Cladosporium (12%), Alternaria (2%) and others (2%). The obtained results showed that fungal concentrations in the present study were nearly high and these conditions should be considered as a risk factor for patients and other persons in the hospital.
In hospital facilities Indoor Air Quality (IAQ) is a critical factor in preventing infections. Unpleasant hospital IAQ may lead to hospital-acquired infections, sick hospital syndrome, and various occupational risks . A large number of studies have showed that various percentages of hospital infections were caused by fungi, such as Candida albicans and diverse species of Aspergillus, Cladosporium, and Penicillium. [2–5]. Fungal air quality in the hospital environment affected by various factors, such as the presence of construction activity and a favorable microclimate. Since exposure to fungi can cause serious health problems, it is clearly essential, in the above-mentioned risk situations, to evaluate the level of contamination in the hospital environments and to use those evaluations to determine the risk of infection for patients and staff alike, in that the use of air conditioning systems does not provide complete protection against fungi [2, 6–8]. Various studies have investigated the fungal air quality in hospital environments. Perdelli et al.  studied various environments in 10 hospitals and found the average concentration of airborne fungi was 19 ± 19 cfu/m3. Fungal air quality of three hospitals was studied in Greec by Panagopoulou et al., and reported that the median concentration of airborne fungi was ranged between 5.5 and 10.6 cfu/m3 . In other study conducted by Li and Hou , Bioaerosol characteristics were investigated in hospital clean rooms. They found that the concentrations of fungi varied from 0 to 319 cfu/m3.
The aim of the present study was to evaluate the level of airborne fungal contamination in various hospital rooms in Shariati Hospital, Tehran, Iran.
Materials and methods
The present study was conducted in ten rooms of Shariati Hospital in city of Tehran, Iran. The air sampling occurred during January to April 2012, and 120 samples were examined. In the hospital, ten rooms in two floors (first and second) were studied and categorized into: Operating Rooms (OR) (8 rooms: General Operating Room 1(GOR1), Orthopedics Operating Room (OOR), Nerves Operating Room (NOR), General Operating Room 2 (GOR2), Urology Operating Room (UOR), Women Operating Room (WOR), Emergency Operating Room (EOR), Maxillofacial Operating Room (MOR)) and Nursing Stations (NS) (2 stations).
Sample collection was performed in respiratory height (about 1.5 m) for 2 minutes using an Andersen one-stage viable impactor (Quick Take-30, SKC, USA) at an airflow rate of 28.3 L/min [10–12], and SKC BioStage single-stage viable cascade impactors equipped with 100 mm diameter Petri dishes containing Sabouraud dextrose agar medium, supplemented with chloramphenicol. Before the air sampling, the head was cleaned with 70% alcohol . The Petri dishes were closed and delivered to the Central Laboratory of Public Health School, Tehran University of Medical Sciences. Indoor air temperature and relative humidity were measured using a digital PHB-318.
Fungi incubation and identification
This step was according to other study  in brief, the plates were incubated at 25°C and counted after 72-120 hours and reported as colony forming unit (cfu/m3). When suspect colonies were detected, they were isolated with plates containing Sabouraud plus chloramphenicol medium. The incubation temperature was 25°C. The airborne fungi concentrations were identified using both microscopic and macroscopic methods for each colony isolated.
Descriptive statistics by the SPSS software version 20 are used for data analysis in this study.
Descriptive statistics for total detected airborne fungi and environment conditions of hospital rooms
No. of Samples
Mean (cfu/m3) (Min-Max)
Indoor environment conditions (Mean)
Concentration of airborne fungi genera in various hospital rooms (Mean ± SD) in cfu/m 3
44 ± 57
18 ± 1
144 ± 276
35 ± 1
18 ± 1
35 ± 1
33 ± 21
35 ± 1
35 ± 1
46 ± 32
90 ± 1
35 ± 1
85 ± 108
36 ± 1
38 ± 21
106 ± 1
18 ± 1
65 ± 107
18 ± 1
15 ± 1
53 ± 1
51 ± 41
28 ± 9
35 ± 12
39 ± 19
53 ± 1
53 ± 49
18 ± 1
41 ± 35
53 ± 1
62 ± 62
18 ± 1
Total mean (first and second floor)
59 ± 34
47 ± 30
34 ± 18
27 ± 12
35 ± 18
To investigate the fungal air quality, 120 samples were collected from ten rooms of Shariati hospital. The results showed that total concentration of detected fungi in the hospital rooms was 55 ± 56 (mean ± SD) cfu/m3. Also the obtained results showed the most frequent fungal genus isolated were Penicillium, Aspergillus and Cladosporium that is consistent with other studies [2, 14, 15].
Fungal air quality in hospital environments was investigated by the numerous researchers. In a study carried out by Sautour et al., in France indoor fungal contamination was conducted during 18-month. They found that the average concentration of viable fungi in indoor air was 4.2 cfu/m3 and the most frequently detected airborne fungi were Penicillium spp. (27- 38%) and Aspergillus spp . Thus the average concentration of fungi reported in their study is much lower than those detected in the present study. Fungal contamination (Penicillium and Aspergillus) of hospital rooms has been investigated in Lithuania and air borne fungi were ranged from 26 to 78 cfu/m3 .
The results of the present study show various levels of contamination in all the hospital rooms, even though all areas are equipped with air conditioning systems. Such contamination may be caused or exacerbated by a range of factors, such as noncompliance with procedural norms (e.g., the frequent opening of doors among the operating theater and the outer environment) and inefficient operation or inadequate maintenance of the air conditioning system, which can allows unfiltered outside air to enter the operating theater. Overall, the mean concentrations of airborne fungi measured in the present study were higher than those recorded in other studies [6–8]. Moreover, our results show that the concentration of airborne fungi is significantly high in rooms and seems this concentration is important risks factor for patients and staff in these rooms. According our observations opening doors and windows are the most important factors that cause these high concentrations of airborne fungi in the rooms. Construction and demolition at the hospital campus during the study is other factor to high concentration of airborne fungi.
In hospital environments indoor air quality is an important factor in preventing infections. Levels of airborne fungal contamination in various hospital rooms in Shariati Hospital, Tehran, Iran have been investigated. The results showed that fungal concentrations were high and these conditions should be considered as a risk factor for patients and other persons in the hospital.
The authors gratefully acknowledge the financial and technical support provided by the Tehran University of Medical Sciences, Tehran, Iran.
- Wan GH, Chung FF, Tang CS: Long-term surveillance of air quality in medical center operating rooms. Am J Infect Control 2011, 39: 302–308. 10.1016/j.ajic.2010.07.006View ArticleGoogle Scholar
- Perdelli F, Cristina ML, Spagnolo AM, Dallera BS, Ottria G, Grimaldi M: Fungal contamination in hospital environments. Infect Control Hosp Ep 2006, 27: 44–47. 10.1086/499149View ArticleGoogle Scholar
- Faure O, Fricker-Hidalgo H, Lebeau B, Ambroise-Thomas P: Eight-year surveillance of environmental fungal contamination in hospital operating rooms and haematological units. J Hosp Infect 2002, 50: 155–160. 10.1053/jhin.2001.1148View ArticleGoogle Scholar
- Fox BC, Chamberlin L, Kulich P, Rae EJ, Webster LR: Heavy contamination of operating room air by Penicillium species. Identification of the source and attempts at decontamination. Am J Infect Control 1990, 18: 300–306. 10.1016/0196-6553(90)90229-LView ArticleGoogle Scholar
- Panagopoulou P, Filioti J, Farmaki E, Avgi MM, Roilides E: Filamentous fungi in a tertiary care hospital: environmental surveillance and susceptibility to antifungal drugs. Infect Control Hosp Ep 2007, 28: 60–67. 10.1086/508832View ArticleGoogle Scholar
- Panagopoulou P, Filioti J, Petrikkos G, Giakouppi P, Anatoliotaki M, Farmaki E: Environmental surveillance of filamentous fungi in three tertiary care hospitals in Greece. J Hosp Infect 2002, 52: 185–191. 10.1053/jhin.2002.1298View ArticleGoogle Scholar
- Rainer J, Peintner U, Poder R: Biodiversity and concentration of airborne fungi in a hospital environment. Mycopathologia 2001, 149: 87–97. 10.1023/A:1007273131130View ArticleGoogle Scholar
- Molina RT, Garijo MA, Munoz RA, Palacios IS: Pollen and spores in the air of a hospital out-patient ward. Allergol Immunopath 2002, 30: 232–238. 10.1016/S0301-0546(02)79126-XView ArticleGoogle Scholar
- Li CS, Hou PA: Bioaerosol characteristics in hospital clean rooms. Sci Total Environ 2003, 305: 169–176. 10.1016/S0048-9697(02)00500-4View ArticleGoogle Scholar
- Naddafi K, Jabbari H, Hoseini M, Nabizadeh R, Rahbar M, Younesian M: Investigation of indoor and outdoor air bactrial density in Tehran subway system. Iranian J Environ Health Sci Eng 2011, 8: 383–388.Google Scholar
- Obbard JP, Fang LS: Airborne concentrations of bacteria in a hospital environment in Singapore. Water Air Soil Pollut 2003, 144: 333–341. 10.1023/A:1022973402453View ArticleGoogle Scholar
- Yang CS, Heinsohn PA: Sampling and analysis of indoor microorganisms. Hoboken, New Jersey: John Wiley and Sons, Inc; 2007.View ArticleGoogle Scholar
- Hoseini M, Jabbari H, Naddafi K, Nabizadeh R, Rahbar M, Yunesian M, Jaafari J: Concentration and distribution characteristics of airborne fungi in indoor and outdoor air of Tehran subway stations. Aerobiologia 2012, 1–9.Google Scholar
- Gorny RL, Dutkiewicz J: Bacterial and fungal aerosols in indoor environment in Central and Eastern European countries. Ann Agric Environ Med 2002, 9: 17–23.Google Scholar
- Tormo-Molina R, Gonzalo-Garijo MA, Fernandez-Rodriguez S, Silva-Palacios I: Monitoring the occurrence of indoor fungi in a hospital. Rev Iberoam Micol 2012, 29: 227–234. 10.1016/j.riam.2012.04.002View ArticleGoogle Scholar
- Sautour M, Sixt N, Dalle F, LOllivier C, Calinon C, Fourquenet V: Prospective survey of indoor fungal contamination in hospital during a period of building construction. J Hosp Infect 2007, 67: 367–373. 10.1016/j.jhin.2007.09.013View ArticleGoogle Scholar
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