Determination of hydrogen cyanide concentration in mainstream smoke of tobacco products by polarography
© Mahernia et al. 2015
Received: 10 February 2014
Accepted: 11 July 2015
Published: 29 July 2015
There has been a worldwide concern for the health risks of cigarette smoking and hydrogen cyanide (HCN) considered as one of the hazardous tobacco compounds which is needed to be determined in order to reduce the dose related to smoke disease risk.
In this study, we prepare the experimental procedure to entrap the HCN from mainstream smoke of different brands of Tehran cigarette, through simulating human inhalation and determine its concentration applying polarography.
The HCN level of the 50 commonly consumed tobacco products (47 cigarettes and 3 cigars) obtained from local store is ranged between 17.56 ± 1.02 and 1553.98 ± 0.56 μg per stick, this acquired amount is more than FDA approval (10 μg per stick), so the harmful effects of smoking is indicative.
The comparative study of the results shows that the price and the weight of each product do not indicate HCN level. As can be seen, R2 value which is a statistical measure of how close the data are to the fitted regression line is low (R2 < 0.2). So it should not be deceived by names such as ultra light or infinite gravity to suck, because this names or the price haven’t effect on the amount of HCN and its destructive effects.
KeywordsTobacco products Cigarette Hydrogen cyanide Polarography
Smoking is the major risk factor of mortality in the world according to the statistical information; the cigarette consumption during one century has increased over 100 times, which increases the concern over the safety of tobacco products [1, 2].
Tobacco smoke contains more than 5000 chemical compounds which 150 of these substances have been proved to be toxicants [3, 4]. Hydrogen cyanide is one of the tobaccos smoke poisonous substances which are formed from the combustion of the protein and nitrate compounds existed in tobacco at high temperatures in the oxygen deficient condition [5, 6] which it’s chronic and low exposure causes neurological, respiratory, cardiovascular and thyroid effect [7–9].
The tobacco smoke pathway includes the part directly entered the mouth called mainstream and the part diffused in the surrounding, called side stream, which hazardous for nonsmoker. The level of HCN in mainstream smoke is ranged from 10 to 400 μg per cigarette (US Brands) which 0.6 to 27 % of these amounts exist in side stream smoke . In the previous study the level of HCN in the non-filtered cigarette was between 400 to 500 μg per stick  and in another study the amount of HCN in the mainstream smoke of cigar, non-filtered cigarette and filtered cigarette was 1035, 59 and 448 μg per 1 g of tobacco, respectively and in small cigars it was between 510 to 780 μg per 1 g of tobacco .
There are different reported methods determine HCN in different samples including voltammetry , fluorometry [14, 15] gas chromatography , LC-MS-MS , HPLC-MS , potentiometry , spectrophotometry  and colorimetry . Among them, polarography the subclass of voltammetry is the most precise and inexpensive method based on the oxidation and reduction mechanism .
The aim of this study is focused on the determination of the HCN in mainstream smock of different brands of cigarette consumed in Tehran using polarography method.
Materials and methods
Collection of samples
Hydrogen cyanide concentration and price of different brands of cigars
Cyanide concentration (μg/cig.)
Number of cig. per pocket
The price of each pocket($)
Weight of each cig. (mg)
The price of each cig. ($)
CAFÉ CRÈME (AROME)
CAFÉ CRÈME (BLUE)
ESSE Special Gold
Marlboro Gold (Germany)
Marlboro (Extra) (USA)
Marlboro Lights (USA)
Marlboro Lights (Switzerland)
Marlboro Lights (Switzerland)
Marlboro Ultra Lights (Switzerland)
Pall Mall (Blue)
Pine (supper slims)
Winston Blue (Europe)
Winston Lights (Imported)
Winston Lights (USA)
Winston Ultra Lights (USA)
Winston Ultra Lights (USA)
Winston Ultra Lights (Switzerland)
Reagents and chemicals
All chemicals used were of analytical reagent grade from Merck (Germany). Buffer solutions were prepared by dissolving boric acid (0.2 M) and potassium hydroxide (0.17 M) in 1000 ml ultrapure water and adjusting the solution to pH 10.2. Cyanide standard solution (1 g/L) was prepared by dissolving 0.2503 g KCN in 100 ml KOH 0.01 M in ultrapure water.
Analysis was conducted by the Metrohm Polarography device 797 VA Computrace, three electrode systems consisting of a dropping mercury electrode (DME) as the working electrode, an Ag/AgCl reference electrode and platinum counter electrode. The device outfitted in the following conditions: stirrer speed 2000 rpm, mode DP, purge time 300 s, equilibration time 5 s, pulse amplitude 50 mV, start potential 0 V, end potential −500 mV, voltage step 8 mV, voltage step time 0.8 s, sweep rate 10 mV/s, peak potential CN −240 mV. All instrumental settings were those recommended in the manufacturer’s manual book and the instrumental conditions with the method of AB110-Det of cyanide . All potentials quoted were measured against an Ag/AgCl reference electrode and the polarographic cell volume was 20 ml.
Method of analysis
All statistical analyses were performed using the statistical software for social sciences (SPSS Inc. Chicago, IL, Version 21). Statistical analyses of each sample were characterized by mean ± standard deviation. The mean levels of cyanide were compared across categories of price, and weight of each cigarette or cigars. The significance level was defined at 0.05 for the regression equations.
Result and discussion
The result of mainstream smoke HCN determination by polarography (Table 1) showed that among 50 samples the average amount of HCN was 184.825 μg per stick which the highest level of HCN pertain to Jewel sweet cigar, 1553.98 ± 0.56 μg per cigar, and the lowest was Swiss Marlboro with 17.56 ± 1.02 μg per cigarette. Moreover, the average amount of HCN in cigarettes and cigar were 218 μg and 785.09 μg, respectively, In addition the average weight of cigarette and cigar samples was 800 mg and 5500 mg, respectively, and the average weight of whole samples was 1084.24 mg.
The changes in the HCN levels are dependent on the preparation procedure of tobacco, which lead to the diverse amount of proteins and nitrate compounds of cigarette which render the conversion of HCN level emission. In addition it may be possible to assess the quality of cigarette especially ultralight by determining the amount of HCN as it is increased in the unfeigned products.
In this study, HCN of the toxic components of the tobacco smoke was determined by polarographic method. The results shows that the ranges of HCN level of 50 samples were varied between 17.56 ± 1.02 - 1553.98 ± 0.56 μg per stick. This acquired amount is more than FDA approval (10 μg per stick).
We gratefully acknowledge the financial support from Tehran University of Medical Sciences.
- General S. Reducing the health consequences of smoking. 25 years of progress. US Department of Health and Human Services. 1989.Google Scholar
- Shafey O, Eriksen M, Ross H, Mackay J. The Tobacco Atlas. 3rd ed. Atlanta, GA: Am Cancer Soc; 2009.Google Scholar
- Fowles J, Dybing E. Application of toxicological risk assessment principles to the chemical constituents of cigarette smoke. Tob Control. 2003;12:424–30.View ArticleGoogle Scholar
- Green CR, Schumacher JN, Llovd RA, Rodgman A. Comparisons of the composition of tobacco smoke and the smokes from various tobacco substitutes. Beit Tabakforsch Intl. 2007;22:257–89.Google Scholar
- Norman V, Ihrig A, Larson T, Moss B. The effect of some nitrogenous blend components on NO/NOx and HCN levels in mainstream and sidestream smoke. Beitr Tabakforsch. 1983;12:55–62.Google Scholar
- Baker R. Smoke Chemistry, Tobacco production. Blackwell Publishing Ltd: Chemistry and technology; 1999. p. 398–439.Google Scholar
- Blanc P, Hogan M, Mallin K, Hryhorczuk D, Hessl S, Bernard B. Cyanide intoxication among silver-reclaiming workers. JAMA. 1985;253:367–71.View ArticleGoogle Scholar
- Chandra H, Gupta B, Bhargava S, Clerk S, Mahendra P. Chronic cyanide exposure biochemical and industrial hygiene study. J Anal Toxicol. 1980;4:161–5.View ArticleGoogle Scholar
- El Ghawabi SH, Gaafar MA, El-Saharti AA, Ahmed SH, Malash KK, Fares R. Chronic cyanide exposure. a clinical, radioisotope, and laboratory study. Br J Ind Med. 1975;32:215–9.Google Scholar
- Fiksel J, Slimak MW, Little AD. An Exposure and Risk Assessment for Cyanide. Monitoring and Data Support Division, Office of Water Regulations and Standards. US: Environmental Protection Agency; 1981.Google Scholar
- Hoffmann D, Hoffmann I. Risks of tobacco smoke exposure are similar for all sources of tobacco smoke, and the magnitude of the risks experienced by cigar smokers is proportionate to the nature and intensity of their exposure. Chem and Toxicol. 1998;55.
- Adams PI. Combustion temperatures in cigars and cigarettes, A comparative study. Tobacco Sci. 1968;12:144–50.Google Scholar
- Safavi A, Maleki N, Shahbaazi H. Indirect determination of cyanide ion and hydrogen cyanide by adsorptive stripping voltammetry at a mercury electrode. Anal Chim Acta. 2004;503:213–21.View ArticleGoogle Scholar
- Lundquist P, Rosling H, Sörbo B, Tibbling L. Cyanide concentrations in blood after cigarette smoking, as determined by a sensitive fluorimetric method. Clin Chem. 1987;33:1228–30.Google Scholar
- Chen D, Castro M, Valcarcel M. Flow-through sensor for the fluorimetric determination of cyanide. Talanta. 1990;37:1049–55.View ArticleGoogle Scholar
- Brunnemann KD, Yu L, Hoffmann D. Chemical studies on tobacco smoke. XLIX. Gas chromatographic determination of hydrogen cyanide and cyanogen in tobacco smoke. J Anal Toxicol. 1977;1:38–42.View ArticleGoogle Scholar
- Lacroix C, Saussereau E, Boulanger F, Goulle J. Online liquid chromatography-tandem mass spectrometry cyanide determination in blood. J Anal Toxicol. 2011;35:143–7.View ArticleGoogle Scholar
- Tracqui A, Raul J, Geraut A, Berthelon L, Ludes B. Determination of blood cyanide by HPLC-MS. J Anal Toxicol. 2002;26:144–8.View ArticleGoogle Scholar
- Bark L, Higson H. A review of the methods available for the detection and determination of small amounts of cyanide. Analyst. 1963;88:751–60.View ArticleGoogle Scholar
- Surleva AR, Visualizing DG, Hazard S. A simple spectrophotometric determination of hydrogen cyanide in cigarette smoke and filters. J Chem Edu. 2013;90:1654–7.View ArticleGoogle Scholar
- Fowler E, Steele TW. The determination of anionic surface-active agents in dilute aqueous solution. Johannesburg: National Institute for Metallurgy; 1968.Google Scholar
- Surleva A. Recent achievements in toxic cyanide monitoring: review. Revue electronique internationale pour la science et la technologie. Numéro. 2009. p. 3.Google Scholar
- Polarographic determination of free cyanide No, 110/2 e. In: Metrohm application bulletin
- Determination of Hydrogen Cyanide in Sidestream Tobacco Smoke. Health Canada. 1999.Google Scholar
- Diekmann J, Biefel C, Rustemeier K. Analysis of cigarette mainstream smoke for 1,1-dimethylhydrazine and vinyl acetate by gas chromatography–mass spectrometry. J Chromatogr Sci. 2002;40:509–14.View ArticleGoogle Scholar
- National Poisons Information Service (NPIS) Hydrogen cyanide. Toxbase®. 2000.Google Scholar
- Lam KK, Lau FL. An incident of hydrogen cyanide poisning. Am J Emerg Med. 2000;18:172–5.View ArticleGoogle Scholar
- Agency for Toxic Substances and Disease Registry (ATSDR). Draft Toxicological Profile for Cyanide. Atlanta. US: US Department of Health and Human Services; 2004.Google Scholar
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