Composting of rice straw with effective microorganisms (EM) and its influence on compost quality
© Jusoh et al; licensee BioMed Central Ltd. 2013
Received: 4 February 2013
Accepted: 5 February 2013
Published: 7 February 2013
This study aims to assess the effect of EM application on the composting process of rice straw with goat manure and green waste and to evaluate the quality of both compost treatments. There are two treatment piles in this study, in which one pile was applied with EM and another pile without EM. Each treatment was replicated three times with 90 days of composting duration. The parameters for the temperature, pH, TOC and C/N ratio, show that decomposition of organic matter occurs during the 90-day period. The t-test conducted shows that there is a significant difference between compost with EM and compost without EM. The application of EM in compost increases the macro and micronutrient content. The following parameters support this conclusion: compost applied with EM has more N, P and K content (P < 0.05) compared to compost without EM. Although the Fe in compost with EM is much higher (P < 0.05) than in the compost without EM, for Zn and Cu, there is no significant difference between treatments. This study suggests that the application of EM is suitable to increase the mineralization in the composting process. The final resultant compost indicated that it was in the range of the matured level and can be used without any restriction.
KeywordsEffective microorganisms (EM) Rice straw Composting Nutrients contents Heavy metals contents
Rice (Oryza sativa) is the most important staple food for a large part of the world’s human population, especially in East and South Asia, Latin America, the Middle East, and the West Indies. It is the grain with the second-highest worldwide production, after maize (corn) . In Malaysia, it is the main source of carbohydrate and covers about 10% of total plantation area. Besides producing rice seed, it also produces a large amount of waste by-product of which one is rice straw residue. It is estimated that from 700,000 hectares of rice plantation area in Malaysia, two million metric tonnes of rice straw will be produced in every planting season . A major portion of this agricultural waste is disposed of by burning or is mulched in the rice fields. These wastes, if not properly handled, will cause many problems to farmers as well as to the environment. If rice straw is left in the field without proper management, it can cause the spreading of disease, which originates from the rice straw, such as stem disease and can also encourage the breeding of pests, especially rats. Burning is not the best way to deal with such waste, as it is harmful to the environment.
The abundance of rice straw as an organic waste can be converted to fertilizer throughout the process of composting. According to Tiquia and Tam , composting is a biological treatment that is cost-effective to treat different types of organic waste. Composting was the first concept for using effective microorganisms (EM) in environmental management. Crop residues and animal wastes have been effectively composted to produce biofertilizers. EM was developed by Professor Dr Teruo Higa in the 1970s at the Ryukyus University, Okinawa, Japan. Basically, this microbial solution was developed for natural or organic farming systems, however, with further research its uses have been expanded to resolve some environmental issues, through which it facilitates the reuse of most waste . Research in Holland  and in Costa Rica  has highlighted that there is potential for compost from the waste of animals or crops from which the results are increasing yields of crops supplied with this material compared to the output of traditional organic systems. Composting of rice straw using effective microorganisms as an accelerator to speed up the composting process and increased nutrients in the compost has not been well documented. Therefore, it is essential to study the effect on the physical and chemical parameters during the process.
The aim of this study is to assess the effect of EM application on the composting process of rice straw with goat manure and green waste and also to evaluate the nutrient and heavy metals at the end of composting. This study is based on the hypothesis that the application of EM on rice straw will increase the microbial activity and thus, increase the composting rate. It also increases the mineralization process of compost through the enhanced microbial colonization and activity. To test these hypotheses, compost from rice straw residue was produced with applications of EM and compared to non-EM treatment.
Materials and methods
Composting process and sampling
In this study, rice straw is used as the main material for composting together with goat manure and green waste (vegetable and fruit waste from the market). The shredded rice straw was soaked in the water for 24 hours before the composting process. All of the raw materials were analysed for physiochemical, nutrients and heavy metals. Composting was carried out in a shaded area at the premises of Premium Agro Products Sdn. Bhd. In this experiment, there are two treatments—compost piles with EM (C1) and compost piles without EM (C2) for control. The mixtures used for all piles were arranged with the ratio as follows: 50% rice straw + 30% goat manure + 20% green waste, in which10 kg of rice straw was mixed with 6 kg of goat manure and 4 kg of green waste, and each treatment was replicated three times. In this study we used commercial EM (EM•1®), which contains lactic acid bacteria, yeast and phototrophic bacteria. The EM solution is required for the production of EMAS. EMAS is actually an activated EM suspension in a mixture of molasses (sugar cane) and non-chlorinated water or rice rinse water (which provides the minerals for the multiplication of the microorganisms). For the activation of EM · 1®, one part EM · 1® microbial inoculants and one part of molasses were mixed with 20 parts of chlorine-free water. This solution was then stored for three to five days in an air tight expandable container for fermentation. Built up gas was released once daily.
For piles with inoculation with EM, a 5% EM solution was applied to constitute 20% of the amount of water to be added to a given mixture. Water was then added until the moisture content reached 60% (wet basis) in each composting mixture. To retain the moisture and prevent excessive loss of heat, the heaps of composting material were then covered using plastic sheets. The moisture content was maintained at 50–60% by the addition of water throughout the active composting period by frequent checking. The mixtures were turned at 3-day intervals to maintain porosity and were composted for 90 days. The temperature was measured daily with a digital thermometer at random depths.
Compost samples were taken from each treatment at 0, 15, 30, 45, 60 and 90 days of the composting and were analysed for changes in the physical and chemical properties throughout the composting process. The samples taken from the piles were divided into two portions. One portion was dried to constant weight (60°C for 2 days) for chemical analysis. The dried samples were ground in mortar to pass through a 2 mm sieve and were stored in screw capped jars. The other portion was analysed for pH and total carbon.
Moisture content was determined using the gravimetric method. Five grams of compost was dried in an oven for 24 h at 105°C (weighed and re-weighed until a constant weight was reached). The samples were then allowed to cool at room temperature before the final weight was taken. The preparation for pH was conducted according to Sundberg et al. and the pH was determined using a digital electrode pH meter. The total carbon was determined by loss of weight using the ignition method and the total nitrogen was determined using the Kjeldahl method . The Total Organic Carbon (TOC) was determined by wet digestion in K2Cr2O7 with concentrated H2SO4, digested on a 150°C preheated block for 30 minutes, and titrated for excess Cr2O2−7 with ferrous ammonium sulphate after cooling. The contents of the mineral elements for Zn, Cu and Fe were determined using the ISO11466  standard method (aqua regia digestion method) and analysed by Atomic absorption spectroscopy (AAS). Determination of P and K was conducted using the In-House Method at ESPEK Research and Advisory Services.
Statistical analysis was carried out by using Statistical Package for the Social Sciences (SPSS) 17.0. All results were tested for normal distribution and then analysed by using the paired-sample t-test.
Chemical and physical properties of raw feedstock (dry weight basin)
7.60 ± 0.08
7.10 ± 0.08
6.50 ± 0.48
39.20 ± 0.95
35.60 ± 1.20
15.30 ± 0.91
61.30 ± 2.61
13.00 ± 1.11
8.40 ± 0.21
11.43 ± 0.77
58.00 ± 0.84
79.00 ± 1.35
0.64 ± 0.07
2.55 ± 0.28
2.90 ± 0.35
0.21 ± 0.02
0.24 ± 0.03
0.18 ± 0.04
1.12 ± 0.11
1.81 ± 0.15
0.95 ± 0.06
Zn (mg kg−1)
38.40 ± 2.20
65.20 ± 2.03
64.30 ± 0.51
Cu (mg kg−1)
9.30 ± 0.67
19.30 ± 0.67
1.32 ± 0.26
Fe (mg kg−1)
129.20 ± 1.90
1003.00 ± 12.49
145.20 ± 1.70
The increase in temperature during the composting process was caused by the heat generated from the respiration and decomposition of sugar, starch and protein by the population of microorganisms. The increment in temperature is a good indicator that there is microbial activity in the compost pile, as a higher temperature denotes greater microbial activity . The temperature pattern showed that there is a rapid progress from the initial mesophilic phase to the thermophilic phase for both these treatments, which points to a high proportion of readily degradable substances, e.g. vegetables and fruits (contained in a green waste material). According to Haug , the composting temperature has to be above 55°C for three consecutive days to kill the pathogen. Although both the compost pile treatments met the requirement, pile C1 has the longest period of time above 55°C, that is, for 6 consecutive days, while C2 only remained for three consecutive days. Even though no microbial test was done on the sample, it can be assumed that the compost treatment with EM (C1) has high microbial activity compared to C2 based on the high temperature achieved.
The declining TOC value, which was 49% for C1 and 36% for C2, is similar to that reported by Benito et al.. According to Tumuhairwe et al., large TOC losses suggest pronounced microbial activity in the former. The t-test indicated that the EM application had a significant difference (p < 0.05) on the compost, which means that treatment C1 lost more carbon (C) than C2 during the composting process. Rice straw is a material that consists of high C content. Diaz et al. reported that during composting, C is a source of energy for microorganisms to build up cells. Almost all of the C is absorbed by the microorganisms and transformed to CO2 during the metabolism process of the cells. The left over C will be changed into membrane and protoplasm form. Throughout the composting process this organic matter is decomposed by microorganisms through which the organic carbon will be oxidized in aerobic condition to CO2 gas to the atmosphere and thus lower the C/N ratio.
The increase in the total nitrogen (N) contents at the end of the composting process differs from the values obtained by Tognetti et al. who found that total N decreases overtime. According to Viel et al. the increase in total N may be due to the dry mass net loss as the loss of organic C as CO2 during composting. In addition, the N values might also increase due to the nitrogen-fixing bacteria activity that commonly occurs at the end of composting . Although a decrease of N can occur due to leaching of NO−3-N and ammonia volatilization, in this experiment, both piles were covered with plastic to retain the moisture and avoid moisture from outside, which could lead to the result obtained. Moreover, in high technology composting, where they can control the leaching problem, the resultant compost achieves an increment in total N [23, 24]. The dependent pair t-test done on the treatments showed that the application of EM also had a significant (P < 0.05) positive effect on the net mineralization of N. The increase in the N value at the end of the composting period might occur due to the usage of N by microorganisms to build up cells, thus reducing the N, and some of the organisms will eventually die, which will be recycled as N and thus contribute to the increase . The increased amount of N at the end of composting is due to this stored source of N.
The phosphorus (P) content decreases in both treatments where the P value in the C1 compost dropped by 4.3% while C2 dropped by 19%. These results are in accordance with other previous reports [26–28]. According to Tumuhairwe et al., the loss of P during the composting process is possibly due to the leaching of P in the soluble organic solute. In contrast, there are other reports that obtain an increase in P value [29–32]. From these studies, there is a significant (P < 0.05) difference between the two treatments. Although both piles decrease in P value, the final output of C1 treatment has the potential to produce compost with a P value higher than the C2 treatment.
K is known as the element that is easily leached out . However, in this study the K value increased to 55% in the C1 compost compared to 17% in C2, which shows that the compost with EM are significantly (P < 0.05) higher than without EM. The use of rice straw as a medium that can absorb moisture and maintain its structural integrity and porosity, might avoid the loss of K in compost . Both treatments (C1 = 1.7; C2 = 1.4) pass the recommended value of K in compost, which is 1%. K plays an important role in plant growth where its function is to increase the elongation of the root, control ion balance, improve protein synthesis, encourage enzyme reaction, and improve the photosynthesis process and food development .
The accumulation of heavy metal (Zn and Fe) increases during composting, except for Cu whereas the values decrease proportional to the time. The increase of Zn and Fe are in line with previous research done by Paré et al.. According to both reports, during composting the accumulation of nutrient and heavy metals significantly increased, which indicates the maturity of the product. Pare et al. also concluded that the stability of biosolid compost can be correlated with the accumulation of heavy metals and nutrients, and, thus extractability and exchangeability of heavy metals.
In both treatments, the final Zn value increased but varied between the initial days until the end. A small increment was achieved by these two treatments, which are only 2% for C1 and 6% for C2. However, both composts are in the safe limit set by the European Standard  for heavy metals in compost, that is, in the range of 210–4000 mg kg−1. The decrease in Cu values in this study is similar to the result obtained by Md Sabiani . Both treatments show that the decline of Cu is proportional to the days of composting, however, for the C2 treatment, there is an increase for day 45 before it decreases again, which differs from pile C1where the Cu increases a bit at the end of the composting period. The Fe values increased in both treatments, and it was found that the Fe contained in C1 was much higher with a range of 560–2624 mg kg−1 during the composting process. This could occur due to the longer exposure to high temperature above 55°C for C1 compared to C2. The high temperature increases the loss in moisture content and C in C1 whereas in C2, the Fe accumulation is low with the range of 650–2379 mg kg−1. The low temperature achieved during the composting process compared to C1 contributed to the low accumulation of Fe. This happened due to the final resultant compost still being high in moisture content, which encourages the leaching process to occur, and, thus, reduces the Fe value. This theory correlates with the finding of Md Sabini  in which compost that loses its moisture content and C accumulate more Fe. The Fe also increases when there is a reduction of substrate pH in the early composting period, which increases the solubility of metals, including metallic microelements . The t-test conducted on the heavy metals showed that there is no significant difference between the treatment with EM and without EM on the value of heavy metals except for Fe. The compost applied with EM shows a significant (P < 0.05) difference in Fe accumulation.
Compost usually contains heavy metals based on their initial raw material. Generally, these heavy metals (micronutrients) are required by the plant for perfect growth. The heavy metals present in the compost, such as Zn, Cu and Fe, are absorbed by plants during the fertilizing process. In a small quantity, these trace elements are necessary for plant growth but in large quantities they can cause phytotoxicity . Kabata-Pendias and Pendias  also described in their research that Zn, Cu, Mn and Fe are useful as trace elements for crop growth. However, constant and intense application of organic compost containing heavy metals will lead to accumulation in the soil and an increase in toxic levels.
In general, all the composting parameters show that both treatments have a similar pattern. All the parameters measured indicate that a decomposition process occurs in both treatments. The parameters for the temperature and pH show that the decomposition of organic matter occurs during the 90-day period. The decrease in TOC values and C/N ratio also shows that an organic compound is being consumed by microorganisms. The t-test conducted shows that there is a significant difference between the compost treated with EM and the compost without EM. The application of EM in compost increased the macro and micronutrient content. The following parameters support this conclusion: compost applied with EM has more N, P and K content (P < 0.05) compared to compost without EM. While the Fe in compost with EM is much higher (P < 0.05) than compost without EM. However, for Zn and Cu, there is no significant difference between treatments. All of the micronutrient (heavy metals) values are below the standard limit of heavy metals in compost.
The author gratefully acknowledges receipt of the National Science Fellowship (NSF) from the Ministry of Science, Technology and Innovation, Malaysia.
- (Food and Agriculture Organization of the United Nations), Production statistic. http://faostat.fao.org/site/567/DesktopDefault.aspx#ancor,
- BERNAMA, Petani digalakkan jual jeramipadi untuk hasil tambah nilai. http://web6.bernama.com/client/fama/newsbm.php?lang=&id=250311,
- Tiquia SM, Tam NFY: Characterization and composting of poultry litter in forced-aeration piles. Process Biochem. 2002, 37: 869-880. 10.1016/S0032-9592(01)00274-6.View ArticleGoogle Scholar
- Sangakkara UR: The technology of effective microorganisms – case studies of application. http://www.emnz.com/index.php/file/display/26/,
- Van Bruchem J, Lantinga EL, Overvest J, Jovink JPM: Environmental tuning of agriculture in the Netherlands. Proceedings of the 5th International Conference on Kyusei Nature Farming: 1998; Bangkok. Edited by: Senanayake YDA, Sangakkara UR. 1999, 233-245.Google Scholar
- Shitani M: Organic fertilizer – Managing banana residues with Effective Microorganisms. Proceedings of the 13th International Scientific Conference of IFOAM: 28 to 31 August 2000; Basel. Edited by: Alfoeldi T, Lockerezt W, Ninggli U. 2000, 08-28.Google Scholar
- Sundberg C, Smars S, Jonsson H: Low pH as an inhibiting factor in the transaction from the mesophilic to thermophilic phase in composting. Bioresour Technol. 2004, 95: 145-150. 10.1016/j.biortech.2004.01.016.View ArticleGoogle Scholar
- Alban LA, Kellogg M: Methods of soil analysis as used in the OSC soil testing laboratory. Agricultural experiment station. 1959, Corvallis: Oregon State CollegeGoogle Scholar
- International Organization for Standardization: ISO 11466-Soil Quality-Extraction of Trace Elements Soluble in Aqua Regia. 1995, Switzerland,Google Scholar
- Iranzo M, Cañizares JV, Roca-Perez L, Sainz-Pardo I, Mormeneo S, Boluda R: Characteristics of rice straw and sewage Sudge as composting materials in Valencia (Spain). Bioresour Technol. 2004, 95: 107-112. 10.1016/j.biortech.2004.01.013.View ArticleGoogle Scholar
- Fujio Y, Kume S, Ueda S: Correlation between physical and chemical changes of rice straw as a major constituent under composting. Jpn Ferment Technol. 1986, 64: 351-354. 10.1016/0385-6380(86)90131-7.View ArticleGoogle Scholar
- Abdelhamid MT, Horiuchi T, Oba S: Composting of rice straw with oilseed rape cake and poultry manure and its effects on faba bean (Vicia faba L.) growth and soil properties. Bioresour Technol. 2004, 93: 183-189. 10.1016/j.biortech.2003.10.012.View ArticleGoogle Scholar
- Fogarty A, Tuovinen O: Microbiological degradation of pesticides in yard waste composting. Microbiol Rev. 1991, 55: 225-233.Google Scholar
- Makan A, Assobhei O, Mountadar M: Effect of initial moisture content on the in-vessel composting under air pressure of organic fraction of municipal solid waste in morocco. Iran J Environ Healt. 2012, 10: 3-View ArticleGoogle Scholar
- Roca-Peréz L, Martinez C, Marcilla P, Buloda R: Composting rice straw with sewage sludge and compost effects on the soil-plant system. Chemosphere. 2009, 75: 781-787. 10.1016/j.chemosphere.2008.12.058.View ArticleGoogle Scholar
- Tumuhairwe JB, Tenywa JS, Otabbong E, Ledin S: Comparison of four low-technology composting methods for market crop waste. Waste Manag. 2009, 29: 2274-2281. 10.1016/j.wasman.2009.03.015.View ArticleGoogle Scholar
- Diaz LF, Savage GM, Eggerth LL, Golueke CG: Composting and recycling municipal solid waste. 1993, United State of America: Lewis PublishersGoogle Scholar
- Haug RT: Compost engineering: principles and practice. 1980, Michigan: Ann Arbor Science PublishersGoogle Scholar
- Benito M, Masaguer A, Moliner A: Chemical and microbial parameters for the characterization of the stability and maturity of pruning waste compost. Biol Fert Soils. 2003, 37: 184-189.Google Scholar
- Tognetti C, Mazzarino MJ, Laos F: Improving the quality of municipal organic waste compost. Bioresour Technol. 2007, 98: 1067-1076. 10.1016/j.biortech.2006.04.025.View ArticleGoogle Scholar
- Viel M, Sayag D, Andrre L: Compost: production, quality and Use. Optimisation of agriculture industrial waste management through in-vessel composting. Edited by: Bertoldi I. 1987, M. Essex: Elsevier Applied Sciences, 230-237.Google Scholar
- Bishop PL, Godfrey C: Nitrogen transformations during sludge composting. BioCycle. 1983, 24: 34-39.Google Scholar
- Bernal MP, Paredes C, Sánchez-Monedero MA, Cegarra J: Maturity and stability parameters of compost prepared with a wide range of organic wastes. Bioresour Technol. 1998, 63: 91-99. 10.1016/S0960-8524(97)00084-9.View ArticleGoogle Scholar
- Goyal S, Dkull SK, Kapoor KK: Chemical and biological changes during composting of different organic wastes and assessment of compost maturity. Bioresour Technol. 2005, 96: 1584-1891. 10.1016/j.biortech.2004.12.012.View ArticleGoogle Scholar
- Polprasert C: Organic waste recycling. Technology and management. 1996, West Sussex, England: IWA PublishingGoogle Scholar
- Tiquia SM, Wan JHC, Tam NFY: Extracellular enzyme profiles during co- composting of poultry manure and yard trimmings. Process Biochem. 2001, 36: 813-820. 10.1016/S0032-9592(00)00281-8.View ArticleGoogle Scholar
- Perkinson R, Gibbs P, Burchett S, Misselbrook T: Effect of turning regime and seasonal weather conditions on nitrogen and phosphorus losses during aerobic composting of cattle manure. Bioresour Technol. 2004, 91: 171-178. 10.1016/S0960-8524(03)00174-3.View ArticleGoogle Scholar
- Ogunwande GA, Osunade JA, Adekalu KO, Ogunjimi LAO: Nitrogen loss in chicken litter compost as affected by carbon to nitrogen ratio and turning frequency. Bioresour Technol. 2008, 99: 7495-7503. 10.1016/j.biortech.2008.02.020.View ArticleGoogle Scholar
- Vourinen AH, Saharinen MH: Evolution of microbiological and chemical parameters during manure and straw co-composting in a drum composting system. Agric Ecosyst Environ. 1997, 66: 19-29. 10.1016/S0167-8809(97)00069-8.View ArticleGoogle Scholar
- Sommer SG: Effect of composting on nutrient loss and nitrogen availability of cattle deep litter. Eur J Agron. 2001, 14: 123-133. 10.1016/S1161-0301(00)00087-3.View ArticleGoogle Scholar
- Iyengar SR, Bhave PP: In-vessel composting of household wastes. Waste Manag. 2005, 26: 1070-1080.View ArticleGoogle Scholar
- Tai HS, He WH: A novel composting process for plant wastes in Taiwan military barracks. Resour Conserv Recy. 2007, 51: 408-417. 10.1016/j.resconrec.2006.10.006.View ArticleGoogle Scholar
- Md Sabiani NH, Asaari F: Kajian perbandingan kualiti kompos daripada sisa tanaman menggunakan pelbagai kaedah pengkomposan. 2004, Pulau Pinang: Universiti Sains MalaysiaGoogle Scholar
- Paré T, Dinel H, Schnitzer M: Extractability of trace metals during co-composting of biosolids and municipal solid wastes. Biol Fert Soils. 1999, 29: 31-37. 10.1007/s003740050521.View ArticleGoogle Scholar
- Brinton WF: Compost quality standards & guidelines: an international view (final report). 2000, New York: Woods End Research Laboratory, Inc.Google Scholar
- Gorski R, Kleiber T: Effect of effective microorganisms (EM) on nutrient content in substrate and development and yielding of roses (Rosa x hybrida) and gerbera (Gerbera jamesonii). Ecol Chem Eng. 2010, 17: 506-512.Google Scholar
- Whittle AJ, Dyson AJ: The fate of heavy metals in green waste composting. Environmentalist. 2002, 22: 13-21. 10.1023/A:1014563807198.View ArticleGoogle Scholar
- Kabata-Pendias A, Kabata F: Trace element in solid and plant. 1992, Florida: CRC PressGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.